Procedure I – Group 1: Known Sample Solutions   Complete the table…

Procedure I – Group 1: Known Sample Solutions

 

Complete the table below using data from Procedure I. For each sample solution (A-E) indicate any color change that occurs when a test solution is added. Fill in the last column (Macromolecule Classification) using data found under the Activity Form tab.

 

Data Table I

 

 

Known Sample Solution	Test  Solution 1 Reaction	Test  Solution 2 Reaction	Test  Solution 3 Reaction	Test  Solution 4 Reaction	Test  Solution 5 Reaction	Macromolecule Classification
A	Color changed (red)	No color change	No color change	No color change	No color change	Sugar
B	Color changed (red)	Color changed (Purple)	No color change	No color change	No color change	Starch
C	No color change	No color change	Color changed (Green)	No color change	No color change	fat
D	No color change	No color change	No color change	Color changed (Purple)	No color change	protein
E	No color change	No color change	No color change	changed (Purple)	Color changed (Grey)	Amino acid

 

 

 

 

 

 

 

 

Observations and Questions

 

[1] Write  hypothesis statement for the experiment conducted for Procedure I.

 

 

 

 

 

 

[2] Which variable represents the independent variable? What information helped  to come to that decision?

 

 

 

 

 

 

 

[3] Would  characterize this experiment as qualitative or quantitative? What information helped  to come to that decision?

 

 

 

 

 

 

 

[4] Refer to completed Data Table I and place the appropriate answer to each of the statements below.

 

Answers: amino acid, starch, fat, sugar or starch, protein or amino acid

 

Statements:

 

Test Solution 1 reacts with:

Test Solution 2 reacts with:

Test Solution 3 reacts with:

Test Solution 4 reacts with:

Test Solution 5 reacts with:

 

 

 

 

 

 

 

 

Procedure II – Group 2: Unknown Sample Solutions

 

Complete the table below using data from Procedure II. For each sample solution (A-E) indicate any color change that occurs when a test solution is added. Next, use Data Table I to determine the macromolecule classification for each sample solution.  Hint: compare the color changes in both tables to make determinations.

 

 

Data Table II

 

 

 

Unknown Sample Solution	Test  Solution 1 Reaction	Test  Solution 2 Reaction	Test  Solution 3 Reaction	Test  Solution 4 Reaction	Test  Solution 5 Reaction	Macromolecule Classification
A	Color changed (Red)	No change	No change	No change	No change	sugar
B	No change	Color changed (purple)	Color changed (Green)	No change	No change	starch
C	No change	No change	No change	Color changed (purple)	No change	fat
D	No change	No change	No change	Color changed (purple)	Change (Grey)	protein
E	Color changed (Red)	No change	No change	No change	No change	Amino acid

 

 

Observations and Questions

 

[5] In Procedure II, did any of  unknown sample solutions (A, B, C, D, E) contain sugars? If yes, which one? What information helped to come to that decision?

 

 

 

 

 

 

 

 

 

 

 

 

[6] In Procedure II, did any of unknown sample solutions (A, B, C, D, E) contain protein? If yes, which one? What information helped you to come to that decision?

 

 

 

 

 

 

 

[7] Using the laboratory tests in this experiment, would  be able to distinguish between a small molecule such as amino acid from a larger molecule such as a protein? Explain why or why not.

 

 

 

 

 

 

 

[8] Using the laboratory tests in this experiment, would  be able to distinguish between a starch and a sugar such as glucose? Explain why or why not.