Explore how to evaluate the appropriate use of tests for different populations based on scales, norms, and score comparability. You also calculate mean, standard deviation, percentile, z-score, and T-score for a dataset.

Explore how to evaluate the appropriate use of tests for different populations based on scales, norms, and score
comparability. You also calculate mean, standard deviation, percentile, z-score, and T-score for a dataset.

 

One of the most useful qualities of a well-designed test is that it allows you to compare an individual to a
population. For instance, when you took the SAT, you found your percentile relative to other college-bound high
school seniors. A test of depression could show how an examinee’s depression compares to the general U.S. population,
to mental health outpatients, or to persons diagnosed with major depressive disorder. A test of job aptitude could
compare a job applicant to successful and unsuccessful job candidates.

explore how to evaluate the appropriate use of tests for different populations based on scales, norms, and score
comparability. You also calculate mean, standard deviation, percentile, z-score, and T-score for a dataset.

•Evaluate appropriateness of tests in terms of scales, norms, and score comparability for different populations
•Calculate mean, standard deviation, percentile, z-score, and T-score

Stress Management by Meditation


An application of social cognitive theory


Introduction

Meditation is about balancing your inner and outer world. The power of meditation is felt rather than read. As per the RAJA YOGA meditation Guide developed by Brahma kumaris in 1994, when your attention is focused more on your surroundings than in your inner qualities, your situations become stronger than you which distract you and make you feel lack of concentration and clarity.

Social cognitive theory is based on observational learning. Social cognitive theory focus on the person’s self-regulation to achieve goal directed behaviour. It considers the unique way in which a person acquire and maintain behavior over a period of time, while also considering the social environment that influence a person to perform the behavior. The theory considers person’s past experiences which determine the probability of adoption of behaviour.

Meditation is good for relieving stress and anxiety as many researches have shown but I was not


able

[SS1]

to perceive that behaviour because I am living in an environment where I cannot spend time for myself. The study stress and workload was affecting my eating habits as well. So I thought I should start doing meditation which was again a challenging behaviour for me as I was thinking, I would not be able to spend time for myself and focus living in a small 1bedroom room apartment with so much of work load. Therefore I selected meditation as a challenging behavior to help me lowering my mental stress and keeping my self away from environmental influences.

In this regard, There was a need to learn doing meditation and keep myself calm. Social cognitive theory would help forming strategies to improve Behavioral Capability, Observational Learning, Expectations, Self-efficacy and self-control to perform meditation regularly. As per the theory, I could learn better if I observe a model doing same behaviour.


Looking in to literature, there is no study with a meditation intervention applying social cognitive theory

[SS2]

but there is a published research study in MEDLINE data base by Mehta and Sharma (2012) who have conducted a Yoga intervention of 10 week to reduce anxiety using the social cognitive theory based strategies. Though I could not find full text study, looking in to abstract I found meditation and yoga almost similar behaviours and chose the social leaning theory to plan my behaviour challenge 3 week intervention.


Broad Objective:

To adopt a healthy lifestyle behaviour by engaging in 15 minutes of meditation every day with a view to manage stress.


Specific Objectives:

  1. To help balance hormonal levels of my body.
  2. To obtain more focus in my study than my busy personal life.
  3. To improve my self-efficacy, concentration and peace of mind.
  4. To feel the joy of the thoughtless state obtained during meditation.
  5. To help myself getting free from emotional attachments.


Methods and Tools:

strategies were made using social cognitive theory to perform the behaviour and tools were selected to measure the outcome. An alarm was set in the cellphone to remind me of performing the behaviour and a record was kept every day after performing the behaviour in the record keeping sheet attached on the wall in my bedroom where I performed meditation every day.

Strategies for learning meditation:

  1. Download and read guide by brahma kumaris-how to perform meditation
  2. Watch online video of meditation

Strategies for performing behaviour:



  1. Choose a Convenient Time
  2. Choose A Quiet Place
  3. Sit in a comfortable posture
  4. Keep a relatively Empty Stomach

    [SS3]


  5. Choose one object to focus i.e. the video of meditation (

    https://www.youtube.com/watch?v=YHDQBSWXqZg

    )
  6. Listen the audio instructions for meditation

    [SS4]


Measurement of outcome:

A scale was developed to rate the self- efficacy from 0-100. Examples of everyday possible difficult situations were written on the scale and marked any applicable situation followed by rating.

The scale used for measuring self-efficacy is given below:


Rate your self-efficacy

Rate your degree of confidence by recording a number from 0 to 100 using the

Scale given below:

0 102030405060708090100

Cannot Moderately Highly certain

do at allcan docan do


Mark the applicable situation you felt for today:

When I am feeling tired _____

When I am feeling under pressure from study _____

During or after experiencing personal problems _____

When I am feeling depressed _____

When I am feeling anxious _____

When I feel physical discomfort when I meditate _____

When I have too much work to do at home _____

When there are other interesting things to do _____

When I have other time commitments _____

After experiencing family problems _____

As a self-monitoring, everyday record was kept for the following outcomes by rating them out of 10.

  1. Rate your concentration: how much you achieved today
  2. Rate your breathing rhythm
  3. Rate your mental calmness after meditation
  4. State any barrier in your performance


Barriers or challenges:

In total I did not perform the meditation for 4 days. The reasons were following:

  • I was feeling sleepy due to my menstrual cramps.
  • I was very busy with my submission and could not even afford to spend half an hour to perform meditation followed by record keeping
  • I was very happy and did not feel like doing meditation
  • I just focused on breathing for 5minutes to save my time

Whereas in all other days I performed meditation because it was really helping me. I was feeling very relaxed after doing it at least for a night. Record keeping was a challenging because sometimes I had to think how much successful I was in my behaviour? An alarm at 10 o’clock was very annoying for my husband in the weekends because he had to leave the bedroom while I perform meditation. I did not find so much challenging to do meditation because I started enjoying it any may be because I knew I had to make a report and I need to record my points to make a good report. Though it is challenging on the previous day of any submission, I have planned to continue this behaviour as much as possible throughout my master’s program. Overall the experience of meditation is great because it taught me to love and care for myself which I forgot after getting married and living with my in laws. Meditation helped me spend time for myself alone in the room even when my husband is at home. That was because he knew it is a part of my submission Mediation help feeling an inner ability that you are the best and you have power to do every task. I was feeling so positive after doing meditation. I did meditation 2times before my facilitation in Nick’s class and I was so confident.


Application of my experience as a Nutrition communicator:

Since my behaviour is more of changing lifestyle, it can be applied as an intervention to reduce stress and anxiety in someone’s everyday life. This behaviour can be very beneficial for working women, university students and those who suffer with anxiety and sleep disorders. If meditation is performed on a regular basis for a longer time, it regulates your hormonal imbalances and change your overall personality and mood. This behaviour can also be useful for people who tend to eat more when they are stressed. So indirectly meditation would help losing weight. As a nutrition communicator I would recommend not only healthy eating but also healthy lifestyle which regulate an overall metabolism of the body. If the behaviour is challenging to adopt, I would recommend to learn and perform using Video because the music and audio instructions help focusing and learning and saves time because a person can do it at home without joining classes or workshop.


Utility of theory and strategies to change my behaviour:

A social cognitive theory helped me creating strategies to perform and learn the meditation. For example, choosing a convenient time and quite place driven me to perform the behaviour every day. It improved my self-regulation in my home environment. I performed behaviour at night around 10pm because I study every night until late and meditation helped me focusing more in study and I taught me how to keep myself away from the social life. A strategy of focusing on video and listening to their instructions helped me learn meditation and focusing. Focusing without a music was very difficult as I tried it on the first observational day of my behaviour. A record keeping helped me measuring my self- efficacy and also it was a motivation to reach to a maximum rating.


References

Mehta, P., & Sharma, M. (2012-13). Evaluation of a social cognitive theory based yoga intervention to reduce anxiety. International Quarterly of Community Health Education. 32(3), 205-217.

Raja Yoga. (1994). Meditation step by step guide by brahma kumaris available at

http://brahmakumaris.info/download/BK%207%20Days%20Course/RY-Meditation-Teachers-Guide-1994.pdf

retrieved on 17th September 2014

(

https://www.youtube.com/watch?v=YHDQBSWXqZg

)



[SS1]

Rational of my behaviour


[SS2]

Application of theory in my behaviour with example of similar research


[SS3]

First 4 strategies help improving self-regulation over behaviour


[SS4]

Last 2 strategies help develop

Observational learning

An Exploratory Study of Student Nurses’ Perceptions of Gender

An Exploratory Study of Student Nurses’ Perceptions of Gender

Though they may be half a world away, nursing schools in India face problems similar to those in the United States when it comes to recruiting men. The following study sought to discern the opinions of 78 senior nursing students studying in and around Pondicherry, India, regarding gender roles in their field. It aimed to determine the following:

Correlation Between Family and Domestic Violence


Assessment Task – 1

The following essay is a discussion of the correlation between family and domestic violence and impacts this has on mental health. Authoritative journal articles which form the basis of evidence-based practice will be used to discuss the impact of domestic violence on mental health taking into consideration bio psychosocial factors influencing domestic violence and mental health for adults and children. This essay will discuss nurses role that identified holistic and consumer-oriented recovery approach by using effective therapeutic communication and it will be also focusing on nursing professional boundaries that recognize a range of nursing assessment and legal responsibility.

Domestic violence is one of the main factors of mental health issues that affect the psychosocial well- being of thousands of families a year (Healey, 2014). Domestic violence is defined as occurring when an abused person within an intimate relationship is intimidated, threatened or controlled by the abusing partner (O’Brian et al, 2013). Domestic violence includes emotional, psychological, physical, sexual and economic abuse (Healey, 2014). Amnesty International Australia (2013) identified that in the majority of cases, women and children are the victims of domestic violence. Children experience trauma witnessing violence in the family, which can have a prolonged psychological effect (Amnesty International Australia, 2013) for example, children living where they witness that their father abusing the mother on daily basis, can result in an aggressive mentality towards women. O’ Brian et al., (2013) stated that domestic violence can also occur between female on male, and between same-sex couples. There are many types of family structure including single parent families, same sex parents’ families, nuclear families and blended families. For the purpose of this essay, the main focus will be on the correlation between on the family members and domestic violence and the impact this has the mental health of the mother, father, and child. According to O’Brian et al., (2013) men are more likely to be the act of continuous, physical and sexual abuse whereas women are more likely to exhibit emotional abuse. In terms of the impact of domestic violence on mental health, there is a consensus among the literature which indicates similar feelings and experiences of men and women which include fear and loss of feelings of safety, feelings of guilt and shame, anxiety, unresolved anger, loneliness and isolation and depression. Although, the physical and psychological issue is common in domestic violence, psychological abuse has long-term adverse effect on victim’s well-being (Ramsay et al., 2012). In domestic violence studies, physically and sexually assaulted women have a high rate of depression which leads to post-traumatic stress disorder (PTSD) (Lacey et al., 2013). Most of the studies mainly focus on female victim; the main reason behind violence against men goes unreported due to fear of humiliation from the society and lack of available support group for men (Dirjber, Reijnders, & Ceelen, 2013). Studies found that men are experiencing less physical assault; however, mostly they face emotional, psychological and verbal abuse (Day et al., 2009). Although, men and women experiencing domestic violence differently; the long-term impact are same which associate with different mental disorder such as dysthymia, anxiety disorder, post-traumatic stress disorder, bipolar affective disorder, depression, schizophrenia and non-affective psychosis (Trevillion et al., 2012). Growing up in the violent environment, children will have less attachment with their parents and antisocial behavior later on their life (Herrenkohl et al., 2011; Bailey & Eisikovits, 2014). Lanius, Vermetten and Pain (2010) stated that physical abuse is one of the major factors of childhood trauma which lead to risk for psychiatric morbidity. The conflict between partners, not only affected their relationship it also impacts on mental status of their children.

Health professional spend more time to assess their patient, which allowed them to identify patients problems and determined to choose the best action for the recovery. Wright, Sparks and O’Hair (2013) have identified that the verity of assessment is the main tool to connect and communicate with the patient to detect key problems. Hungerford et al., (2015) have stated that the assessment determines what patient experiencing and how these experience affecting them. It can be only possible when health professional build therapeutic communication with the patient. A bio psychosocial approach brings together all the aspects to avoid conflicts which consider biological, psychological and social dimensions (Hungerford et al., 2015; Melchert, 2010). According to Sadigh (2013) a bio psychosocial assessment useful to get past and current information, and look for patients’ future condition. Therefore, this identifies patients past and present issues and encourage them to discuss about what changes that they would like to make to recover from those issues. Person-centred care is an approach which involves patient and their families’ decisions to assure most appropriate need (Clissett at al., 2013; Hungerford et al., 2015). When health professional taking person-centred care approaches, they have to consider patients own decision about how they wish to receive. It is also important that health professional should not prejudge to the mental health patient and build trusting relationship by applying therapeutic communication (Hungerford et al., 2015). Nursing consideration is all about gathering more information from the client, by using various types of assessment and communication techniques. It is also important for the nurse to aware patients’ age, gender, religion, employment status and relationship with their family or partners. Townsend (2015) described that a therapeutic interpersonal relationship is the nursing process, where psychiatric nurse focuses to get more information from the patient in various mental health setting. A therapeutic interpersonal relationship classified in four phases: the interaction phase, the orientation phase, the working phase and the termination phase. In preinteraction phase nurse prepare for first meeting with the client, during orientation phase nurse create environment to establish trust with the client, working phase nurse maintain trust promoting clients’ insight and perception, and termination phase evaluate client condition for the further assessment. According to Townsend (2015) while implementing therapeutic interpersonal relationship, nurse must be aware of the boundaries in nurse and client relationship; which includes: materials, social, personal and professional boundaries that allow nurses to recognise acceptable limit. For example, touching clients provide them comfort and encouragement as nature of nursing care; however, nurse must considerate professional boundaries and apply appropriate non-verbal communication. Nurse must maintain the professional relationship towards client instead of personal relationship; i.e. romantic, sexual, or other similar personal relationship is not appropriate between nurse and client. Every individual patient have their own triggers, the nurse must understand and implement accordingly. Although, mental health assessment considers all aspect of the patient, each time it should occur when health professional interaction with the patient (Hungerford et al., 2015). Mental state examination (MSE), clinical risk assessment, and suicidal assessment are the most common assessment mental health (Hungerford et al 2015). Mental state examination is the fundamental factor of patients’ assessment, clinical risk assessment identifies potential risk and minimized the level of risk (Szmukler and Rose, 2013) and suicidal assessment include variety of interventions to assure patients safety and encourage better health. Every state has their own mental health assessment and framework, whereas New South Wales implemented the Mental Health Outcomes and Assessment Tools (MH-OAT) to measure the effectiveness of the health care provided (NSW Health, 2013). MH-OAT includes MSE, substance use, physical examination, family history and development history (Hungerford et al 2015). MH-OAT helps health professionals to work effectively and efficiently by ensuring NSW meet National Standard of Mental Health Care and which provide standard clinical document that include triage, assessment, care plan, review and discharge (NSW Government Health, 2014). According to the Australian Collage of Mental Health Nurses (2013) standard practice provide practical benchmark which minimise level of performance for register nurses in mental health setting; this includes 9 standard practices which are: acknowledging cultural diversity, establishing collaborative partnership, developing therapeutic communication, values other stakeholders contribution, reduce stigma, demonstrate evidence-based practice, practice common law and specialist qualifications. It is very important that nurses must familiar with the legal and ethical context of mental health care. In Australia, each state has its own mental health legislation which known as ‘Mental Health Act (MHA)’; MHA protect as individual and community by emphasising on providing right treatment in least restrictive environment (Hungerford et al 2015).

In conclusion, this essay successfully correlated between family and domestic violence which lead to various mental health problems by giving perfect example of affected family. It discussed major mental health priorities and strategies such as effective therapeutic communications, therapeutic interrelationship, person-centre approach and bio psychosocial approach which reduce conflict and minimize potential risk for themselves and patients. It also explained the importance of the nursing assessments and legislations for the nurses.


References

Amnesty International Australia. (2013, July 19).

Mythbusting violence against women

. Retrieved from

www.amnesty.org.au

.

Bailey, B., & Eisikovits, Z. (2014). Violently reactive women and their relationship with their abusive mother.

Journal of Interpersonal Violence, doi: 10.1177/0886260514549463

, 1-24.

Clissett, P., Porock, D., Harwood, R. H., & Gladman, J. R. (2013). The challenges of achieving person-centred care in acute hospitals: A qualitative study of people with dementia and their families.

International Journal of Nursing Studies, 50

(11), 1495-1503.

Day, A., O’Leary, P., Chung, D., & Justo, D. (2009).

Domestic Violence – Working with Men: research, practice experiences and integrated responses.

Leichardt, NSW, Australia: The Federation Press.

Dirjber, B. C., Reijnders, U. J. L., & Ceelen, M. (2013). Male victim of domestic violence.

Journal of Family Violence, 28

(2), 173-178.

Healey, J. (2014).

Domestic and family violence.

Balmain, NSW: The Spinney Press.

Herrenkohl, T. I., Moylan, C. A., Tajima, E. A., Klika, J. B., Herrenkohl, R. C., & Russo, M. J. (2011). Longitudinal Study on the Effects of Child Abuse and Children’s Exposure to Domestic Violence, Parent-Child Attachments, and Antisocial Behavior in Adolescence.

Journal of interpersonal violence, 26

(1), 111-136.

Hungerford, C., Hodgson, D., Clancy, R., Monisse-Redman, M., Bostwick, R., & Jones, T. (2015).

Mental Health Care: An Introduction for Health Professionals in Australia

(2nd ed.). Retrieved from

http://online.vitalsource.com/books/9780730317487/epubcfi/6/62

.

Lacey, K. K., McPherson, M. D., Samuel, P. S., Sears, K. P., & Head, D. (2013). The Impact of Different Types of Intimate Partner Violence on the Mental and Physical Health of Women.

Journal of Interpersonal Violence, 28

(2), 359-385.

Lanius, R. A., Vermetten, E., & Pain, C. (2010).

The Impact of Early Life Trauma on Health and Disease: The Hidden Epidemic.

United Kingdom: Cambridge University Press.

Melchert, T. P. (2010). The growing need for a unified biopsychosocial approach in mental health care.

Procedia – Social and Behavioral Sciences, 5

(1), 356-361.

NSW Government Health. (2014, August 28).

MH-OAT for Mental Health Professionals

. Retrieved from

http://www.health.nsw.gov.au/mhdao/DM/Pages/professionals.aspx

NSW Health. (2013, October 30).

MH-OAT for Mental Health Professionals

. Retrieved from

http://www.health.nsw.gov.au/mhdao/mhprof_mhoat.asp

O’ Brian, K.L., Cohen, L., Pooley, J. A., & Taylor, M. F. (2013). Lifting the Domestic Violence Cloak of Silence: Resilient Australian Women’s Reflected Memories of their Childhood Experiences of Witnessing Domestic Violence.

Journal of Family Violence, 28

(1), 95-108.

Ramsay, J., Rutterford, C., Gregory, A., Dunne, D., Eldridge, S., Sharp, D., & Feder, G. (2012, Sep). Domestic violence:knowledge, attitudes, and clinical practice of selected UK primary healthcare clinicians.

British Journal ofGeneralPractice, 1

(1), 647-655.

Sadigh, M. R. (2013). Development of the biopsychosocial model of medicine.

Virtual Mentor, 15

(4), 362-365.

Szmukler, G., & Rose, N. (2013). Risk assessment in mental health care: Values and costs.

Behavioral Sciences & the Law, 31

(1), 125-140.

The Australian Collage of Mental Health Nurses. (2013).

Standards of Practice in Mental Health Nursing

. Retrieved from

http://www.acmhn.org/publications/standards-of-practice

Townsend, M. (2015).

Psychiatric Mental Health Nursing: Concepts of Care in Evidence-Based Practice

(8th ed.). Philadelphia, PA: F. A. Davis Company.

Trevillion, K., Oram, S., Feder, G., & Howard, L. M. (2012). Experiences of Domestic Violence and Mental Disorders: A Systematic Review and Meta-Analysis.

PLoS ONE, 7

(12), e51740.

Wright, K., Sparks, L., & O’Hair, D. (2013).

Health Communication in the 21st Century

(2nd ed.). New York: Wiley-Blackwell.

A 25-year-old Arab American man is one day post op following a thoracotomy. There have been no post-surgical complications.

A 25-year-old Arab American man is one day post op following a thoracotomy. There have been no post-surgical complications.

A 25-year-old Arab American man is one day post op following a thoracotomy. There have been no post-surgical complications. He tells his family that he is “in terrible pain”, yet he reports ratings of 2 to 3 on 0-10 numeric rating scale to the nursing staff. He requests pain medication every two to three hours, and will not get out of bed or ambulate.

Identify and state a priority nursing diagnosis label for your assigned patient related to pain.
Develop and state three (3) nursing interventions for this nursing diagnosis label or patient problem. When planning individualized nursing interventions, consider the patient’s type of pain and cultural perspective.
Provide your rationale or reasoning for each intervention chosen.
Base your initial post on your readings and research of this topic.Your initial post must contain a minimum of 250 words. References, citations, and repeating the question do not count towards the 250 word minimum.

What factors contribute to the yearly incidence and mortality rates of various cancers in Americans? What changes in policy and practice are most likely to affect these figures over time.

2. What factors contribute to the yearly incidence and mortality rates of various cancers in Americans? What changes in policy and practice are most likely to affect these figures over time.

Upon entering the site it is clear that there is a hotline posted for any questions or assistance needed. If questions aren’t answered from reading the information that is posted, then the hotline can additionally assist. Immediately the heading labeled “Cancer A-Z” caught my eye. I had a friend that recently died from cancer after being diagnosed one week before. Due to the lack of information of the next steps of cancer, we went to this website for guidance. This site breaks down cancer by its type, explains all about that cancer, treatments, diets, and so on. Not only is it like an encyclopedia for cancer, but also a glossary for unknown medical terms. Under the “Treatment and Support” tab, there are resources for those interested, such as paying, treatment, nearing end-of life, caregiving, and children. All these tools are support systems depending on the topic and needs of the patient. We were able to use the tool on “Treatment & Side effects” to inform our friend about what the next steps would be, even before the doctor saw him! They simply informed him to come back a week later for an informational class on chemotherapy. He never made it.

What services would you recommend and why?

Because, my friend died so quickly, I would recommend really looking into Nearing-End of Life. My friend was 20 years older than his wife and they had no children. Although, many things were handled smoothly, the quick death has left my friend with some tasks she has had to do blind and alone. So, I would recommend everyone look into this category, and the possible outcomes if death occurs. Next, would be caregiver. I believe those caring for loved ones with cancer need help. My sister-in-law’s brother passed away at a young age after being married for 2 years. Due to his brain cancer, it changed him and he had some mood swings. His wife left him, and we believe had she reached out for help to the family and a support system, it would not have had to come to that.

2. What factors contribute to the yearly incidence and mortality rates of various cancers in Americans? What changes in policy and practice are most likely to affect these figures over time.

According to NIH (2017) “SES is most often based on a person’s income, education level, occupation, and other factors, such as social status in the community and where he or she lives. Studies have found that SES, more than race or ethnicity, predicts the likelihood of an individual’s or a group’s access to education, certain occupations, health insurance, and living conditions including conditions where exposure to environmental toxins is most common, all of which are associated with the risk of developing and surviving cancer”. According to NCCS (2017) “ Public policy is a system of regulatory decisions, legislative actions, funding priorities, and other courses of action as well as analysis by advocates and other groups. Everyone who is diagnosed with cancer is impacted by public policy.” Meaning, the impact can include funding for research for certain therapies, screening guidelines, Medicare coverage for treatment, and many more financial and practical issues related to care. Since the beginning of the survivorship movement, policy change has been essential to ensuring cancer survivors have access to high-quality, evidence-based cancer care.

Describe the program and discuss what impact the research will have on the prevention or treatment of cancer.
Road to Recovery is a program that is offered the American Cancer Society (2017). It helps in the following three ways:

Coordinate a ride with an American Cancer Society volunteer driver
Coordinate a ride with a local organization that has partnered with us to provide transportation
Refer you to a local resource you can contact for help
Furthermore, one personal research program that I liked was the Cancer Control and Prevention Research. This program basically studies the behaviors that can reduce cancer risk, iit targets early detection, and can recommend ways improve quality of life of patients and families.

Surgical site infection in patients in hospitals

Background: Surgical site infection (SSI) is highly prevalent in patients undergoing gastrointestinal operations. As patterns of wound infection in these patients are ever changing, SSI surveillance programs periodically to document the trend of SSI rate in these patients are essential. This study was conducted with the primary intention to audit our SSI rate in elective gastrointestinal operations to monitor closely and sustain good SSI rate.

Methods: We audited of patients undergoing elective clean and clean-contaminated gastrointestinal operations at a tertiary-care hospital in Singapore over four year period. The Criteria and definitions of SSI by the Centers for Disease Control, USA were used to identify and diagnose SSI. We analyzed the yearly SSI rates according to wound types and operative procedures for four consecutive years. We also studied the microbiological pattern of infection in these SSI patients.

Results: From 2006 to 2009, there were 5100 patients who underwent elective gastrointestinal operations. Forty-four SSIs were identified, giving an infection rate of 0.9 SSIs/100 operations. Colorectal operations had 2.9% SSI, upper gastrointestinal gave rise to 0.9%, hepatobiliary operations had 0.8% and hernia operations had 0.1%. Among them, 93% were superficial incisional SSI. The commonest microorganisms involved were E coli (29% among infected sites), MRSA (17%), Enterococcus (14%) and Enterobacter (14%).

Conclusion: In conclusion, our hospital surgical infection rates were lower than the average National Nosocomial Infections Surveillance (NNIS) rates. Clinical Practice Improvement Program (CPIP) is effective in sustaining good result. Appropriate management of preoperative, intraoperative, and postoperative wound care and a surveillance system based on international criteria, were useful in maintaining good SSI rates in our hospital and comparison to international data.

CLINICAL AUDIT OF OUR SURGICAL SITE INFECTION RATE FOR ELECTIVE OPERATIONS IN A DIGESTIVE SURGERY UNIT

INTRODUCTION

Surgical Infection is a preventable complication and efforts made to prevent this complication shall be the priority of every patient. Infection at or near surgical incisions within 30 days of an operative procedure, defined as Surgical Site Infection (SSI), contributes substantially to surgical morbidity and mortality. The incidence of SSI ranges from 2% to 5% for patients undergoing surgical procedures each year in the United States, resulting in 500,000 infections, 3.7 million excess hospital days, and $1.6 billion in extra hospital charges. SSI is the second commonest infective complication, accounting for 20% to 25% of the total nosocomial infection [3]. SSI has been well studied in many hospitals worldwide [4-7].

SSI is considered one of the most important problems in the surgical wards. Although complete elimination of infection in surgical patients is impossible, a reduction of its incidence to a minimal acceptable level can result in great benefits for patients and would save economic resources. The etiology of surgical infection is multifactorial, and the necessity to reduce and control it requires surveillance as well as a hospital-wide effort, with institutional support and leadership.

Accordingly, the best strategy in controlling SSIs is in their prevention. This encompasses meticulous operative technique, timely administration of appropriate preoperative antibiotics, and a variety of preventive measures aimed at neutralizing the threat of bacterial, viral, and fungal contamination posed by operative staff, the operating room environment, and the patient’s endogenous skin flora.

In 2005, our hospital statistic indicated that the infection rates for clean operations and clean-contaminated operations were 1.6% and 4% respectively [ ]. Although these figures might have underestimated the SSI rates as some of the SSI cases were not captured by our system, the surgical team felt that there was room for improving in our SSI rates for clean and clean-contaminated operations. We adopted the Clinical Practice Improvement Program (CPIP) Strategy [5] to improve our SSI rates since 2005 by implementing evidence-based practice. In 2010, we conducted a four-year period audit in a tertiary-care hospital in Singapore. We analyzed our SSI rate each year, the incidence of SSI by operative procedure, microbiological pattern in SSI cases and study the risk factors.

Materials and Methods

Our hospital is a 1440-bed tertiary care public institution. We studied all patients from the Digestive Disease Centre who had elective upper gastrointestinal, hepatobiliary and pancreatic, colorectal, abdominal cavity operations and hernia operations. All patients were followed up for 30 days after operations to identify and diagnose SSI.

Our hospital adopted the criteria put forth by the CDC. SSIs are classified as either incisional or organ/space, with incisional SSIs being further subclassified as superficial (involving only skin and subcutaneous tissue) versus deep (involving underlying soft tissue) [ ].

Surgical wounds are classified according to the level of contamination into Class I (Clean wounds), Class II (Clean-Contaminated wounds), Class III (Contaminated wounds) and Class IV (Dirty-Infected wounds).

Clean wound is defined as an uninfected operative wound in which no inflammation is encountered and the alimentary, genital, or uninfected urinary tract is not entered. In addition, clean wounds are primarily closed and, if necessary, drained with closed drainage. Examples are herniorrhaphy and hepatectomy.

Clean-contaminated wound is defined as an operative wound in which the alimentary, genital, or urinary tracts are entered under controlled conditions and without unusual contamination. Specifically, operations involving the biliary tract and gastrointestinal tract are included in this category, provided no evidence of infection or major breach in technique is encountered.

In our CPIP project launched in 2005, the team brainstormed all factors causing SSI and they were summarized in the Fish-Bone Diagram. The factors were broadly classified as environment, staff, equipment, patients and procedural factors. Using the Pareto methodology, four factors are selected based on priority and evidence-based interventions were designed.

Four interventions were introduced and embedded into our new work flow:

(1) Clippers instead of shavers were used for preoperative hair removal. Signs were used as reminder and patients were instructed not to self-shave preoperatively.

(2) Standardized prophylactic antibiotics regime that is consistent with the Ministry of Health guidelines and the Department of Surgery Prophylactic Antibiotic Regimen, and the timing of antibiotics administration within 30 minutes prior to surgical incision were strictly adhered to by anaesthetists.

(3) Individualized glucose monitoring regime for diabetic patients was implemented. Post-anaesthesia care unit (PACU) and ward nursing officers were assigned the responsibility and accountability for monitoring and control of blood glucose level to ensure that the postoperative blood glucose level is below 11.1mmol/l for all diabetic patients.

(4) Postoperative normothermia within the range of 36.0-38.0 degree Celsius was maintained using warmed forced-air blankets preoperatively, intra-operatively and postoperatively in the PACU; in addition to warmed intra-venous fluids, increased ambient temperature in the operating room and electrical warming blankets are placed under the patients on the operating table.

The workflow was redesigned to embed the above new interventions into our workflow. The project was piloted in the fourth quarter of 2005 and fully implemented in January 2006.

Studied population

All patients undergoing elective major operations in the Department of General Surgery were included in the study. Operations under local anesthesia, trauma patients, emergency operations, infected, contaminated and dirty operations and operations of anorectum, oropharynx, skin grafts, burns or scalds were excluded. All ventral hernia, inguinal hernia, upper gastrointestinal, hepatopancreaticobiliary and colorectal operations under the clean and clean-contaminated categories were included.

From January 2006 to December 2009, 5100 patients underwent elective clean and clean-contaminated gastrointestinal and hernia operations in our department. Out of the 5100 patients, 1863 (36%) patients had hernia operations, 1923 (38%) had hepatopancreaticobiliary operations, 860 (17%) had colorectal operations, 344 (7%) had upper gastrointestinal operations and 110 (2%) had operations to abdominal cavity (Figure 1).

There was both laparoscopic and open type of operations in the casemix of our study. Hernia operation included inguinal hernia, ventral hernia and recurrent hernia operations. Hepatobiliary operation included cholecystectomy, choledochoduodenectomy, common bile duct exploration, hepatectomy, pancreatectomy and splenectomy. In colorectal operations, there were anterior resection, abdominoperineal resection, hemicolectomy and colostomy operations. Upper gastrointestinal operations included total or partial gastrectomy, gastrostomy, Ivor-Lewis operation and Heller’s operation. Abdominal cavity operations comprised of exploratory laparotomy, adhesiolysis, resection of retroperitoneal tumour and abdominal sarcoma operations.

According to the data collected from July 2008 to December 2009, the study group comprised 3:2 ratio of male and female patients; median age of the patients was 59 (16-96) years. The median ASA score was II (I-IV) while median duration of operations was 90 mins (15 – 830 mins).

Data Management

Data were collected from January 1, 2006, to December 31, 2009. Electronic Operating Room reservation system was reviewed every day and the operations that met the inclusion criteria were flagged. The medical records of the patients, operative notes, anesthetic records and microbiology investigation data were reviewed. Information on operative procedure such as the type of operation, degree of wound contamination was also recorded. Medical records of discharged patients in the outpatient department and medical records of readmitted patients were also reviewed for evidence of infection that developed after hospital discharge.

Interim data analysis of SSI rate was done quarterly to evaluate the trend of SSI rates. Frequent updates in charts format were visually fedback to all staff members in the OR, PACU and wards. This allowed the staffs to see how their care could positively impact on the patient outcome and thus motivate them to continue the good practice.

Surgical Site Infection Surveillance

Patients with SSI were identified by both inpatient surveillance and post-discharge surveillance. We realized that a few patients might not be captured in our data collection when they were managed at primary care level. To improve our capture rate, we adopted two levels of surveillance. First was the physical examination of the surgical site of all inpatients with SSI by our surgeons, surgical nurse clinicians, or infection control nurses. Second level of surveillance was the detection of outpatient SSI through post-discharge phone contacts to our patients or patient’s primary care providers by our nurse clinicians or doctors. Outpatient SSI surveillance also included examination of the patients’ wounds during follow-up visits. Post-discharge phone review of our patients also helped to minimize default follow up at the clinic. This process improves our SSI pick-up rate.

Medical records of all SSI patients were thoroughly reviewed by the project leader and the team. Data was captured and SSI was confirmed before classifying it according to the criteria of the Centres for Disease Control and Prevention [6]. Any equivocal cases were moderated by a panel of independent reviewers. Wounds were swabbed for microbiological analysis if a purulent discharge was present at the time of review. Microbiology results were interpreted in conjunction with the clinical information as well as inputs from our microbiologists. A positive culture did not necessarily imply infection and a negative result might not necessarily exclude SSI.

Statistical analysis

Incidence of SSI was calculated by dividing the number of infections by the number of operations performed and then multiplied by 100. The frequency of the organisms identified as causative pathogens responsible for infection was calculated by dividing the number of isolates by the number of infections.

Results

Among 5100 patients, there were 44 SSIs identified between Jan 2006 and Dec 2009, for an overall infection rate of 0.9%. During this interval, SSI rates ranges from 0.3% to 1.9% according to quarterly audits (Figure 2). An increase in the SSI rates (spikes) were noticed in the 3rd quarter of 2008 and 4th quarter of 2009; this could be due to the admission of new staff workers who have yet to be familiarized with our guidelines and protocol for SSI prophylaxis.

Among the 44 SSIs, 41 (93%) were superficial SSIs, 1 (2%) was deep SSIs, and 2 (5%) were organ/space SSIs (Figure 4). The patients were followed for 30 days. The number of Superficial, deep and organ/space SSI in each subspecialty was shown in Table 4.

The overall SSI rate was 0.05% in patients with clean surgical sites and 1.3% in clean-contaminated surgical sites for gastrointestinal and hernia operations over four years. The SSI rates in clean operations were 0% in 2006, 2007 and 2009 and 0.2% in 2008. SSI rates in clean-contaminated operations were 1.1% in 2006, 0.7% in 2007, 1.7% in 2008 and 1.8% in 2009 respectively (Table 1).

Analysis by sub-specialty, the overall incidence of SSI within 4 years was 2.9% in colorectal operations, 0.9% in upper gastrointestinal operations, and 0.8% in hepatobiliary and pancreatic operations and 0.1% in hernia operations. There was no SSI in abdominal cavity operations within four years (Figure 3). The yearly SSI rates in subspecialties were shown in the table 2.

In 2005, the year prior to SSI prevention CPIP implementation, the SSI rate was 3.1%, this was used as baseline for comparison. Four years after the bundle of interventions was implemented, 99.1% of the elective patients in this cohort were free from SSI, giving the overall SSI rate of 0.9% for both clean and clean-contaminated elective gastrointestinal and hernia operations. This resulted in an overall relative reduction of SSI by 71% within four years when benchmarked against 2005 data for all clean and clean-contaminated elective gastrointestinal and hernia operations (3.1% vs. 0.9%) (Figure 5).

The SSI rate for clean operations over four years was 0.05% and the overall clean-contaminated SSI rate was 1.3%. This resulted in 97% reduction of SSI in clean operations (1.6% to 0.05%) and 68% reduction in clean-contaminated operations (4% to 1.3%) following implementation of CPIP projects (Figure 6).

SSI Microbiology

In the majority of SSI cases, the pathogen source is the native flora of the patient’s skin, mucous membranes, or hollow viscera.7 When skin is incised, underlying tissue is exposed to overlying endogenous flora.8 Most typically, aerobic gram-positive cocci such as Staphylococcus aureus was the frequent contaminant, with resistant pathogens such as methicillin-resistant S aureus (MRSA) representing an increasing proportion of such infections in recent years.9,10 Surgical entry into hollow viscera exposes surrounding tissue to gram-negative bacilli such as Escherichia coli, gram-positive organisms such as enterococcus, and, occasionally, anaerobes such as Bacillus fragilis.2 There is increasing trend of hospital acquired infection in recent years, for example, ESBL represents the hospital acquired infection. In our study, we analyzed 42 culture results of SSI patients and two of the patients did not have cultures. The commonest microorganisms in our SSI patients were Escherichia coli (29%), followed by Staph aureus (26%). Among colorectal SSI cases, the top three microorganisms involved in their wounds were Escherichia coli, MRSA and anaerobes. In hepatobiliary and pancreatic operations, Enterobacter, Enterococcus and Escherichia coli formed the top three organisms (Table 3). The finding that Escherichia coli was the most common pathogen in our SSI patients was surprising as it was not the skin flora, mostly found in the other studies. However, we didn’t find ESBL in the culture of our patients. In our study, S. aureus was the 2nd most common cause of surgical wound infections accounting for 26% of SSI. Of these, 64% were methicillin resistant. Overall MRSA rate in four year period was 1.4 per 1000 operations. Yearly MRSA rates per 1000 operations were 2.8, 0, 2.2 and 3.5 in 2006, 2007, 2008 and 2009 respectively.

In colorectal SSIs, top three organisms found were E coli, MRSA and anaerobes. In hepatobiliary SSIs, microorganisms such as enterobacter, enterococcus and E coli were the commonest. Among upper GI SSIs, we found that both methicillin resistant and sensitive S. aureus and mixed growth as well.

Palliative Care – Clinical Experience

Introduction

The purpose of this assignment is to reflect on a clinical experience in palliative care and demonstrate the knowledge and skills associated with this. The symptoms, symptom management and government guidelines and standards will also be discussed in relation to how this will impact the care of the patients who are experiencing life threatening illnesses. This will be achieved through using Gibbs (1988) model of reflection. I chose this model of reflection because it is clear and cyclical model which allows the reflector to revisit the same problem and also to examine my practice which will help me to develop and improve in the future.

Description

As an adult nursing student, I will be reflecting on an experience with a patient who was receiving palliative care that I encountered during my community placement. The patient received palliative care and required management of symptoms to help maintain their quality of life for the patient and their family. This involved a patient, who will be named Mr. Jones for confidentiality reasons. Mr. Jones was an elderly patient who had been diagnosed with bowel cancer along with liver metastases, the cancer was in the advanced stages. Mr. Jones was being cared for at home by his wife and carers who visited him at home twice a day to help with getting him up, washing and dressing and helping him back to bed. The district nursing team also visited him on a regular basis. When meeting with the patient his main concern was his pain control and available options which he would be able to receive to manage this.

Feelings

Initially when I heard about the diagnosis I was intrigued and wanted to find out more about the patient and their condition. When I met the patient I felt sympathetic towards him and the family and upon discussion he revealed that he felt like ‘giving up’ and ‘ending it all’. A mixture of thoughts entered my mind, although I could understand why he would want to give up, the only reason was because he was worried about the pain he was starting to experience. When the nurse and I discussed the pain control options he appeared to be more positive about the situation and apologized for what he had said. On reflection it was a positive experience as it allowed me to see how people cope differently with terminal conditions, and the impact it has on the family and carers

Evaluation

During this experience I thought that the nursing team had built a good professional relationship with the patient and their family. The patient had plenty of time to discuss any concerns or issues that he had .The issues discussed such as symptom management were all assessed and prioritized well; how the patient is feeling is important and needs to be taken into consideration. This would also need to be discussed with his wife alone, to find out how she is feeling and to offer her support. When discussed further, the feelings of ‘giving up’ related to his pain management. This is why the Visual Analogue Scale was used for a couple of weeks to monitor the progression of his pain (Crichton 2001). I found the tool to be beneficial for effective management of pain because it was a good indicator as to when we would need to adjust his analgesia using the World Health Organization (WHO) analgesic ladder (WHO, 2004). This aims to give the correct drug, correct dose, given at the correct time and proves to be inexpensive and 80-90% effective (WHO, 2004). This ensured the patient was in the least amount of pain which enabled them to carry on with activities of daily living.

Analysis

The World Health Organization (2009) has defined palliative care as: “an approach that improves the quality of life of patients and their families facing the problem associated with life-threatening illness, through the prevention and relief of suffering by means of early identification and impeccable assessment and treatment of pain and other problems such as physical, psychosocial and spiritual.”Caring for those receiving palliative care in the community during the end stages of life requires an extraordinary commitment from the nursing team, not only human resource but also competence, compassion and focus in anticipating the needs of the patient and family. It is a complex activity involving a holistic approach, building relationships together with expert professional skills and decision making processes (Melvin 2003). As well as pain, other common symptoms during advanced cancer are nausea and vomiting, with rates ranging from 50-60% (Kearney and Richardson, 2006). It is important to treat the reversible causes of nausea and vomiting before resorting to antiemetics. This can be achieved by appropriate assessment and documentation of symptoms which evaluate the effect of treatment given and can be completed and reviewed quickly to utilize less patient energy and nursing time (Kearney and Richardson, 2006). Cancer patients also experience psychological or affective symptoms of fatigue which include lack of energy and motivation, depression, sadness and anxiety, the sense of willpower and ‘fighting spirit’ is often lacking and personal resources that have usually kept a person going in the past are ineffective (Juenger, 2002). Fatigue can take away the ability to do things that the patient and family want to do or need to do. The control over life events reduces, further affecting the person’s quality of life. An essential part of nursing care for fatigue includes returning some of that control (Porock 2003). Giving information can be a positive way to do this. Nurses can explain that the person is not alone in the experience as fatigue is an expected occurrence in advanced cancer and discuss the causes of fatigue, effects and side effects of treatment and the impact of stress and depression, this will help the patient to manage the fatigue more effectively (Porock 2003).Mr. Jones was prescribed oral slow-release Morphine Sulphate for his pain, however he was beginning to experience break through pain, nausea and vomiting which was making it difficult for him to take his morphine. We suggested he kept a record of his pain over a couple of weeks using a visual analogue scale (VAS). When we visited him again we were able to use the VAS to assess his pain and make changes to analgesia as appropriate using the analgesic ladder (see appendix). From the assessment it was appropriate to increase his analgesia to step 3 of the analgesic ladder (WHO, 2004). A syringe driver was prescribed to administer morphine and an antiemetic over a 24 hour period which could be increased or decreased as required. Providing drug compatibilities have been checked, a combination of three drugs can usually be administered safely with the advantage that vomiting will not affect absorption, as it is given subcutaneously and the gut is not involved (Thompson, 2004). Although using a syringe driver improves symptom management with minimal inconvenience, complications can arise and nurses should be able to recognize these quickly and solve the problems efficiently (Lugton, 2002). The disadvantages of using this equipment is the painful injection site, infection risks and infusion rate problems (Thompson, 2004).

Mr. Jones commented on ‘giving up’ and after further discussion, we found this related to his pain. Evidence shows that there is a link between chronic pain and depression as they share similar physiological pathways, whilst social and psychological factors appear to affect the severity of it and when they co-exist the severity of both conditions appears to worsen (Gray, 2001). Ineffective management of psychiatric disorders can be caused by failure to recognize, diagnose and treat appropriately in palliative care settings with 50% of psychiatric disorders being undetected (Payne et al, 2007). Patients with cancer should have access to appropriate psychological support and should be assessed regularly by trained professionals as recommended by National Institute for Clinical Excellence (NICE, 2004).Many people may wish to try complementary and alternative medicines to improve symptoms of their cancer, the reason being many feel dissatisfied with conventional medicine and feel the desire to experience holistic health care for symptomatic relief with a sense of well being (Nayak et al 2003). Auricular acupuncture (acupuncture to the ear), therapeutic touch, and hypnosis may help to manage cancer pain, whilst music therapy, massage, and hypnosis may have an effect on anxiety, and both acupuncture and massage may have a therapeutic role in cancer fatigue (Mansky and Wallerstedt 2006). However there is a lack of evidence to support that complementary and alternative medicine treatments are effective, the risks associated with them are unknown and can potentially be harmful. There has been increasing concern with the use of botanicals and dietary supplements by cancer patients because of the potential interaction between them and prescription drugs (Mansky and Wallerstedt 2006). During this experience, I realised just how important communication is, not only in this situation, but any situation that includes the patient, relatives, carers and multi-disciplinary team. Communication is an essential part of good nursing practice and forms the basis for building a trusting relationship that will greatly improve care and help to reduce anxiety and stress for patients, their relatives and carers (NMC 2008). Groogan (1999) acknowledges that communication is not something that people to do one another, but it is a process in which they can create a relationship by interacting with each other. From Mr. Jones’ perspective, patient-focused communication can be the most important aspect of treatment, due to its capacity to exacerbate or relieve the fear that often accompanies cancer, with evidence of effective communication resulting in decreased anxiety, greater coping ability and adherence to treatment (Dickson 1999). Communication can be divided into three types; cognitive, emotional and spiritual. Cognitive communication involves the giving and receiving of information, emotional communication involves the feeling and expression of psychological responses and spiritual communication involves the expression and feeling of thoughts relating to existing issues beyond the person (Fallowfield & Jenkins 1999). When speaking with Mr. Jones and his family the language used was clear and easy to understand. Stress, emotions and fatigue that accompany a terminal illness make it necessary for the information to be repeated to ensure the patient and family have absorbed it and feel reassured (Latimer 2000).Mr. Jones was given enough time to discuss his concerns and issues without feeling like he had a limited period or that the nurses had many other patients to see, however, what often matters is the quality of interaction rather than the length of time. Giving a few moments of time which are totally focused on the patient’s communication needs can often limit the amount of time spent communicating later when further explanation or clarification is needed (Faull et al, 2005). Mallet and Dougherty (2000) suggested that patients tend to be more dissatisfied with poor communication than with any other aspect of their care and concluded ineffective communication continues to be a major issue in health care.The Department of Health has produced the End of Life Care Strategy (DoH 2008) which promotes high quality care for all adults at the end of life which is the first for the United Kingdom and covers adults in England. Its aim is to provide people approaching the end of life with more choice about where they would like to live and die. In addition, the Macmillan Gold Standards Framework (2003) is another way of combining many different practices. The framework includes 7 Gold Standards which relate to key aspects of care, and guidelines for best practice on teamwork and continuity of care, advanced planning, symptom control and support for patients and their carers. Primary Health Care Teams who join the programme are guided and supported through a combination of workshops, resource materials and networking (Macmillan Cancer Relief 2003). In place is also the NICE clinical guidance on supportive and palliative care (NICE 2004) which advises those who develop and deliver cancer services for adults with cancer about what is needed to make sure that patients, their families and carers, are well informed, cared for and supported. These initiatives had a positive impact on Mr. Jones as the nurses were able to use these guidelines to offer Mr. Jones the best care suited for him and he was then able to make informed decisions regarding the care he would like to receive. These initiatives also have a positive impact on health care professionals as they are supported and encouraged to improve their knowledge through workshops and resource materials.

Action Plan/Conclusion

My reflection of this experience has taught me how complex it is caring for someone receiving palliative care. It requires a holistic approach to ensure the patient and the family receive the best possible care in accordance with the guidelines and standards, in which they must remain empowered and make informed choices regarding their care and treatment with the help of health care professionals. I now understand how complicated symptom management can be; cancer patients experience many symptoms from their condition and also side effects from their medication. Assessments are vital to ensure the appropriate treatment and management of symptoms. The importance of communication has been brought to my attention once again, in my future nursing practice I will focus on my communication skills as this is an essential part of good nursing practice and plays a vital role in palliative care. Although this was an upsetting experience it has also been a positive one as this will affect my future nursing practice a great deal as I have gained vital skills to look after those receiving palliative care and I am now aware of the various strategies and frameworks in place to ensure patients and families receive a high standard of care.

Transposable DNA Elements

Most genes occupy fixed sites on the chromosomes. However, researchers have found that some genes (DNA sequences) can actually change position in the chromosomes. These mobile DNA sequences are called

transposable elements

or simply

transposons

. Typically they are quite small ranging from 500 to 10,000 nucleotide-pairs, but some are larger. Transposable elements are ubiquitous specific DNA sequences of the genome found in both prokaryotes and eukaryotes. At the molecular level, these elements exhibit considerable variation in structure and function. Transposable elements were discovered by Barbara McClintock in maize in 1950.

Transposable elements are generally of two types. One type encodes proteins which induces the transposable element to move directly to a new position or replicate the transposable element to produce a new element that integrates elsewhere in the genome. These types of elements are found in both prokaryotes and eukaryotes. The second type of transposable element is related to retrovirus. Such elements encode a reverse transcriptase for making copies of DNA from their RNA transcripts, which subsequently integrate at new sites in the genome. Such elements are found only in eukaryotes.

In eukaryotes, transposable elements can move to a new position within the same chromosome or to a different chromosome. In prokaryotes, they can move to a new position on the single chromosome present. Transposable elements can inserts into new chromosomal locations without having a homologous sequence; and therefore the process of insertion is different from homologous recombination. Such recombination is called

non-homologous recombination.

Transposable elements can induce various genetic changes. By inserting themselves into the coding region of a gene, they can alter the reading frame or introduce stop codons. If inserted into the regulatory region of a gene, they can disrupt expression of the gene. They can also create double-stranded breaks, inversions and translocations in the chromosomes. Transposable elements have contributed to the evolution of the genomes through induction of chromosomal rearrangements. Usually the frequency of transposition varies with different elements, but it is very low, as higher frequency may lead to lethality.

Insertion sequences (IS) or IS elements is the simplest transposable element found in bacterial chromosomes and plasmids. IS elements were first identified in

E. coli

, from the observations that some mutations affecting the expression of genes, that control the metabolism of the sugar glucose, did not have properties typical of a point mutations or deletions. It was later found that such changes were induced due to insertion of an approximately 800 base-pair DNA segment in to a gene. This particular segment of DNA is called insertion sequence 1, or IS1 (

Fig.6.19

).


E. coli

contains several IS elements (e.g. IS1, IS2 etc), each may present up to 30 copies per genome. Each one of them has a characteristic length (ranging from 768 bp to >5000 bp) and unique nucleotide sequence. All IS elements end with identical or near identical inverted repeats (IR’s) of 9 to 41 bp, but in opposite orientations. For example, the inverted repeats of IS1 consist of 23 bp, but the sequences are not completely identical.

An IS elements can integrate at random locations along the chromosome often inducing mutations, by disrupting either the coding or regulatory sequence of a gene. Promoters present within the IS elements may also effect by altering the expression of nearby genes. IS elements can also induce deletions and inversions in the adjacent DNA thereby causing mutations.

An enzyme called

transposase

, encoded by the IS element, is responsible for transposition of the IS elements. Presences of inverted repeats (IR’s) are essential as transposase recognizes these sequences to initiate transpositions. Insertion of IS element takes place at a target site with which the IS element has no sequence homology (

Fig.6.20

). First, a staggered cut is made in the target site and the IS element is then inserted, and joined to the single stranded ends. Then DNA polymerase and DNA ligase fills-in the gaps. The IS element is thus integrated into the chromosome with two direct repeats (oriented in the same direction) of the target-site sequences flanking the IS element. The direct repeats are called target-site duplications.

The second type of transposons found in prokaryotes is called

Tn transposons

which are similar to IS elements but it contains additional genes. Transposons are of two types; composite transposons and noncomposite transposons. Composite transposons are comparatively complex in nature, having functional genes (e.g. resistance against antibiotics), flanked on both sides by IS elements (

Fig.6.21

). The IS elements are called ISL (left) and ISR (right) and basically of same type, however they may be in the same or inverted orientation depending on the transposon. As the IS’s elements have terminal inverted repeats, the composite transposons also have terminal inverted repeats. Tn10 is an example of composite transposon. The enzyme transposase, produced by one or both IS elements, recognizes the inverted repeats of the IS elements and process of transposition is similar as described for IS element. Composite transpositions produce target site duplications in the new location after transposition.

Noncomposite transposons also contain functional genes like antibiotic resistance, but ARE elements are not present in the terminal ends (example Tn3). They have repeated sequences at genes present in the central region. The enzyme transposase catalyzes the insertion of a transposon into new sites, and the second enzyme, resolvase is involved in the recombinational events associated with transposition. Noncomposite transposons also cause target-specific duplications when moved to a new location.

In

E. coli

transfer of genetic material between two mating types (F

+

and F



) is the result of the function of the fertility factor F. The F

+

strains have the F factor within the cell whereas F



cells does not. F factor is an episome, an extrachromosomal genetic element, which is capable of self-replication and integration into bacterial chromosome. When F

+

strains and F



were co-cultivated, the F factor can move from F

+

strains to F



strain and thereby convert the F



cells to F

+

strain (see

Chapter 4

). F factor contains four IS elements, namely one copy IS2, two copies IS3, and one copy of an insertion sequence element called gamma-delta. The

E. coli

chromosome also has copies of these four insertion sequences at various locations. Thus, integration of the F factor into the main chromosome of the bacteria can be achieved by pairing of the homologous sequences and subsequently exchange of genetic material. Depending upon how the F factor has paired with its homologous segments in the chromosome, integration of the F factor may have different orientations.

While studying black Mexican sweet corn in 1930s, Rhodes observed that when allele

a

of A1 locus is present in homozygous condition, it cannot synthesize purple anthocyanine pigments, and the aleurone layer of the seed remains colourless. However, the presence of Dt (dotted) gene on another chromosome can influence the phenotype of the aleurone layer. Plants of genotype

a/a


Dt/Dt

or

a/a


Dt/dt

produce seeds with dots of purple colour as if allele

a

got mutated to the dominant wild type allele A1. Moreover, the number of doses of the

Dt

allele affected the number of dots – one dose produced on the average 7.2 dots per seed, two doses produced 22.2, and three doses produced 121.9. Rhodes called

Dt

as

mutator gene

, a gene which can increase the spontaneous mutation frequency of another gene.

McClintock carried a series of experiments with maize during 1940s and 1950s that led her to put forward a hypothesis on existence of what she called ‘controlling elements’, which can move freely in the genome and can modify or suppress gene activity. Later the controlling elements she studied were shown to be transposable elements. Recent work has showed that Rhode’s mutator gene

Dt

is also a transposable element.

Presence of transposable elements has been shown in many eukaryotes, which include yeast, fruit fly, maize and human. Structurally and functionally the transposable elements found in prokaryotes and eukaryotes are very similar. Eukaryotic transposable elements have genes that encode enzymes required for transposition, and they can integrate into chromosomes at a number of sites. Effects of such insertions are similar as described for prokaryotic organisms.

The yeast carries a transposable element called

Ty

in its haploid genome, which is about 5.9 kb long and bounded by 340 bp long DNA segment called the delta sequence at each end. Each delta sequence is oriented in the same direction and is called long terminal repeats (LTRs). Sometimes LTRs becomes detached from a

Ty

element, producing solo delta. Each delta contains a promoter and sequences recognized by transposing enzymes. The

Ty

element encodes a single mRNA of 5,700 nucleotides, which begins at the promoter in the delta at the 5’ end of the element (

Fig.6.23

). The mRNA transcript contains two open reading frames (ORFs), designated

Ty

A and

Ty

B. They encode two different proteins required for transposition. On average there exist 35 copies of Ty element in each yeast cells, although the number may vary in different strains.

The genetic organization of the

Ty

elements is similar to that of eukaryotic retrovirus. This single stranded RNA virus synthesizes DNA from their RNA after infecting the cell. The DNA then inserts itself into a site in the genome, where it can be transcribed to produce progeny viral RNA genomes and mRNAs for viral proteins. This inserted material has same overall structure as in yeast

Ty

element.

Ty

elements transpose by making RNA copies of the integrated DNA sequence and then creating a new

Ty

element by reserve transcription. The new

Ty

elements would then integrate at new sites in the chromosome.

Ty

elements have two genes,

A

and

B

, which are analogous to the

gag

and

pol

genes of retrovirus. The two genes produces virus like particles inside yeast cells, but it is not known whether these particles are infectious. Because of their overall similarity to the retroviruses,

Ty

element is also called

retrotransposons

and the process is called

retrotransposition

.

Several families of transposons have been identified in plants. Each family has two forms of transposons – autonomous elements and non-autonomous elements. Autonomous elements can transpose themselves, whereas non-autonomous elements cannot transpose themselves, as they lack the genes for transposition. Therefore, non-autonomous elements require the presence of an autonomous element to carry out transposition. Usually, within the family, the non-autonomous element is derived from autonomous element, with loss of function of one or more genes required for transposition.

When an autonomous element is inserted into a functional gene, the resultant mutant gene is unstable, because the element can excise and transpose to another location. On the other hand, when the non-autonomous element is inserted into a gene, the mutant is stable, because the element is unable to transpose by itself. However, if the autonomous element of its family is present in the genome, it may provide the necessary enzymes required for transposition, and the non-autonomous element also will be able to transpose, making the mutant gene unstable.

While working with maize, Barbara McClintock observed that purple coloured kernel is produced by the wild–type gene

C

, and colourless by the mutant

c

. Sometimes rather than being either of a solid purple colour or colourless, kernels with spots of purple pigment on the otherwise white (colourless) background are produced. After careful genetical studies, she concluded that the spotted phenotype was due to what she called ‘controlling elements’, which is actually transposon. During kernel development, the mutant gene

c

reverts to the wild-type

C

, leading to development of spotted purple pigment. The earlier the reversion occurs, the larger is the purple spot. McClintock found that the

c

(colourless) mutation resulted from a ‘mobile controlling element”, called

Ds

(for dissociation) being inserted into the

C

gene. It is now known that this insertion take place by the transposition of a nonautonomous transposon. A second mobile controlling element called

Ac

(for activator) is required for transposition of

Ds

into the gene.

Ac

can also excise

Ds

out of the

c

gene, producing a wild-type revertant

C

.

Ac

has now been identified as autonomous transposable element.

Molecular basis of

Ac-Ds

system was understood only in 1983. The autonomous

Ac

element is 4,563 base pair long, with a single gene encoding the enzyme transposase and short terminal inverted repeats. Upon insertion into the genome, an 8 base pair direct duplication of the target site is generated.

Ds

elements have all the same terminal inverted repeats (IRs) as

Ac

elements, but are heterogeneous in length and sequence. The variation in length and sequence occur as most

Ds

elements are generated from

Ac

element by deletion of segments or by more complex sequence rearrangements. Because of these variations,

Ds

elements remain transposition-defective and cannot exert transposition activity themselves.

Insertion of

Ac

element takes place through conservative transposition mechanism (cut-and-pest) during chromosome replication (

Fig.6.22

). When a chromosome with one

Ac

element (present at a site called donor site) replicates, two copies of

Ac

will be produced, one on each daughter chromatids. Depending upon which chromatid is involved, there are two possible results of

Ac

transposition, one involving the replicated and other unreplicated chromosome site. If we consider transposition to a replicated chromosome site, and if one of two

Ac

elements transposes to an already replicated site, an empty donor site is left on one chromatid, and

Ac

element remains in the homologous donor site on the other chromatid (

Fig.6.22

). The inserted

Ac

element is inserted into the same chromosome. Thus there is no net increase in the number of

Ac

elements in the chromosome. On the contrary, if the

Ac

element is transposed to a nonreplicated chromosomal site, it will create an empty donor site, but since it was inserted into an unreplicated site it will be duplicated after replication. Thus there will be increase in the number of

Ac

elements in the chromosome. Transposition of

Ds

elements occurs in similar fashion but requires the enzyme transposase encoded by

Ac

elements.

Diabetes Mellitus in the emergency services

Diabetes mellitus is one of the most common medical issues affecting people today. There are two types of diabetes. The first is type one, it is often referred to as juvenile diabetes or insulin-dependent diabetes. Patients with this type of diabetes are often diagnosed with the disorder early in life but on rare occasions, it can be diagnosed as late as forty years of age. People with type one diabetes make up between ten and twenty percent of all diabetics. Men are also more common to be diagnosed with type one diabetes.

While it is not fully known why people develop diabetes it is known that it is a genetic disorder and can be passed down from generation to generation. People with siblings with this disorder increase their chance of developing type one diabetes by six percent. The reason it is often called insulin-dependent diabetes is that the body does not form any insulin from the beta cells of the pancreas, so therefore the patient needs to take daily insulin shots to keep their blood sugar low.

Type two diabetes is often referred to as adult-onset diabetes or non-insulin-dependent diabetes and makes up the remaining eighty to ninety percent of diabetics. Patients with this form of the disorder often do have some type of insulin production by the beta cells in the pancreas but just do not produce enough to maintain in their body. Also in some cases, the patients develop a type of insulin resistance where their body does not use the insulin that is naturally produced by their body correctly or efficiently. In most cases, this type of diabetes can be controlled by a strict diet or the use of oral medications. The diet of a patient with type two diabetes often includes fruits, vegetables, whole grains, and low-fat dairy products. Patients with type two diabetes what to avoid high amounts of sugars, trans-fats, and sodium. When a patient eats large amounts of calories and fat, their body then causes a spike in their blood glucose level. Type two diabetes can sometimes also be reversed with exercise to lose excess weight. People with family members with type two diabetes have a ten to fifteen percent increased risk of developing this disorder.

Two types of issues arise with diabetes. Sometimes the patient’s blood glucose level is too high and sometimes it is too high. When it is too low it is called hypoglycemia. A patient is usually determined to be suffering from hypoglycemia when their blood glucose level is below 60mg/dL. When the body is in a state of hypoglycemia the body automatically slows insulin production and increases glucagon production by alpha cells. Often times hypoglycemia in both type one and type two diabetics is due to overmedication of injected insulin. It can also be caused by exercise, malnutrition, and alcohol consumption. Over time the pancreases’ ability to produce glucagon is sometimes decreased making it harder to raise blood glucose levels during hypoglycemia. When a patient has a high blood glucose level it is called hyperglycemia. A patient is usually considered hyperglycemic if their blood glucose level is above 300 mg/dL.

Hyperglycemia occurs because the body is unable to produce insulin to promote the uptake of glucose from the cells. There are two sub-types of hyperglycemia. The first is diabetic ketoacidosis or DKA. This type of hyperglycemia is most often found in patients with type one diabetes because DKA occurs when there is little or no insulin in the body causing the blood glucose level to soar. It can be caused by untreated type one diabetes or excess glycogen production due to stress. Patients in DKA often present with a blood glucose level above 350mg/dL. Due to the lack of insulin, the body then uses fats as metabolic fuels, and ketoacidosis is developed. The other type of hyperglycemia is called a hyperosmolar hyperglycemic nonketotic coma. This occurs most often in patients with type two diabetes. Because patients with type two diabetes still produce some insulin, unlike in DKA the body is still able to move sugar into the cells and not have to use the fatty tissue from the body. Patients experiencing hyperosmolar hyperglycemic nonketotic coma usually have blood glucose levels of 600 and above.

Diabetes has a large impact on pre-hospital emergency medicine because often when people are having hypoglycemic or hyperglycemic emergencies paramedics are the first to treat these patients. Sometimes when paramedics are dispatched to these calls the caller may not know what is exactly wrong with the patient. Patients in a hyperglycemic or hypoglycemic crisis may present in a variety of ways. The key with these kinds of patients is to keep a high index of suspicion.

As previously stated patients having diabetic issues may present in a variety of ways. Patients suffering from hypoglycemia will often present with hunger, nausea, and weakness. Due to poor cardiac output of the body, the patient will often present with a rapid and weak pulse. The patient will also present with seizures or small twitches. Lastly, the most common symptom is altered mental status. The patient can present in total unconsciousness, drowsiness, confusion, or even aggravated violence.

Change in mental status usually comes with a quick onset because once the body is insulin deprived the brain is the first body structure to suffer because the brain uses glucose as an energy source. When the patient presents with an altered mental status it can mimic many other conditions. The patient can look as if he or she is intoxicated because of their lack of coordination and aggravated attitude. They are sometimes so combative that it may be difficult to effectively assess them. Hypoglycemia can also mimic a stroke. Due to its effect on the nervous system, the patient may present with weakness on one side for no apparent reason.

Lastly, the patient may present like a person experiencing epileptic seizures. The seizures are also a result of the brain’s lack of glucose. These seizures can be of all types; they can be full-body grand maul seizures or partial seizures that only affect certain parts of the body. It is very important that paramedics identify that these symptoms are underlying symptoms of hypoglycemia and treat the true problem correctly. Patients who are experiencing hyperglycemia have some similar symptoms to hypoglycemia but also distinguish symptoms that are different. One of the distinguishing groups of symptoms is the “polys.” These symptoms include polyuria, polydipsia, and polyphagia. Polyuria is defined as excess urination, polydipsia is extreme thirst and lastly, and polyphagia is the feeling of extreme hunger. Like patients experiencing hypoglycemia, these patients will present with tachycardia and altered LOC. One symptom that is specific to DKA is Kussmaul respirations with a fruity smell to their breath. This type of breathing is when the patient had rapid and deep respirations. The reason for this is that the body is breaking down fats due to the lack of insulin the body enters into a state of metabolic acidosis. Deep respiration is the body’s way to “blow off” carbon dioxide to make the body more alkolidic and return the body’s pH level to normal. The patient will also present with a fruity smell on their breath when experiencing DKA. This is due to the ketones being broken down in the body. HHNC’s only distinguishing factor is the lack of Kussmaul respirations and the fruity breath odor.

Many social and ethical issue can and do arise when treating patients with this condition. One social issue is that this problem occurs quite often with homeless and improvised persons. This is because they are often not able to get the proper medications and also not eat proper meals to keep their blood sugar at a normal level. Also these types of patients are sometimes known to drink alcohol and skip out on meals. The combination of malnutrition and alcohol consumption will cause diabetes to worsen significantly. One social and ethical issue is seeing through the symptoms that sometimes indicate other medical problems and determining that the patient is suffering from a diabetic emergency. Patients sometimes present identically to how a person who is severely intoxicated would. These patients can often be rude and violent. It could be very easy to mistake a hypoglycemic patient with an intoxicated individual and have the police transport them to the jail without ever obtaining a BGL check to determine the true problem. This mistake could cause the patient further internally injury and even death. The key with these types of patients is to keep a high index of suspicion. Lastly a common ethical issue is when to let these types of patients refuse care. Often times EMS will arrive and treat the patients symptoms and bring them back to their normal state of consciousness. For many patients it is not the first time the rescue squad has had to respond to their home to reverse their hypoglycemia and do not see a need to go the hospital. The paramedic should try to convince the patient that they should go to the hospital and explain to them the risks of refusing care, but if they still refuse and are alert and oriented the paramedic needs to respect their decision to refuse care and document the event accordingly.

One positive thing about encountering a patient with hypoglycemia prehospitaly is that this issue is usually easily reversed. When treating hypoglycemia you want to first asses the patients Airway, breathing and circulation. Patients in hypoglycemia may have decreased respirations so assisting ventilation with a BMV may be necessary. If the patient is breathing at a normal rate they should have oxygen administer to them via a nasal canula or non-rebreather to combat hypoxia. Once ABCs are taken care a blood glucose reading needs to be obtained from the patient’s finger to confirm that the patient is indeed experiencing hypoglycemia. If the patients BGL is below 60mg/dL first consider 15-30g of oral glucose to be administered only if the patient is conscious and is able to swallow. If IV access can be obtained then 25g of D50 should be administered via IV or IO. If IV access cannot be obtained 1mg of glucagon should be administered IM. The patient should also be hooked up to cardiac monitor to rule out cardiac dysrhythmias. In the case of a patient experiencing hyperglycemia first asses the patients Airway, breathing and circulation. Patients in hyperglycemia may have decreased respirations so assisting ventilation with a BMV may be necessary. If the patient is breathing at a normal rate they should have oxygen administer to them via a nasal canula or non-rebreather to combat hypoxia. Once ABCs are taken care a blood glucose reading needs to be obtained from the patient’s finger to confirm that the patient is indeed experiencing hyperglycemia. If the patient is found to be experiencing hyperglycemia with a BGL of 300mg/dL attempt to obtain IV access and admitter a 250ml/hr fluid bolus with normal saline. This will help combat dehydration associated with hyperglycemia and help “thin out” the glucose enriched blood.

In conclusion diabetes is a true medical emergency. It should be taken very seriously but can often be treated effectively in a pre-hospital setting. Paramedics need to keep a high index of suspicion to make sure they do not misdiagnose patients that are actually having diabetic emergencies.